ABSTRACT
<p><b>OBJECTIVE</b>To investigate the constituents of the Annona squamosa and evaluate their anti-tumor activity.</p><p><b>METHOD</b>The compounds were isolated and purified by various column chromatography. Their structures were elucidated by spectral data analysis. Their anti-tumor activity was assayed by SRB method.</p><p><b>RESULT</b>Eleven compounds were obtained from the 95% EtOH extract. The structures were determined as: annosquamosin C(1),15, 16-epoxy-17-hydroxy-ent-kau-ran-19-oic acid (2),16,17-dihydroxy-ent-kau-ran-19-oic acid(3), annosquamosin A(4), ent-kaur-16-en-19-oic acid (5), 19-nor-ent-kauran4-ol-17-oic acid (6),16-hydroxy ent-kau ran-19-oic acid (7), ent-15beta-hydroxy-kaur-16-en-19-oic acid (8), annosquamosin B (9), ent-16beta, 17-dihydroxykauran-19-al (10), 16, 17-dihydroxy-ent-kauran-19-oic acid me thyl ester (11). Compounds 1,2,3,5,9 showed different inhibitory activities against 95-D lung cancer cells,the effect of compound 5 was strongest with the IC50 value 7.78 micromol x L(-1); Compounds 2, 5, 9 showed inhibitory activities against A2780 ovarian cancer cells, the effects of compounds 2 and 9 were strong with the IC50 values being 0.89, 3.10 micromol x L(-1), respectively.</p><p><b>CONCLUSION</b>Compound 2 was firstly isolated from this family, while compound 8 and 10 were first found from this genus and the title species, respectively. The in vitro anti-tumor test showed compound 5 significantly inhibited 95-D lung cancer cells and compounds 2 and 9 exhibited remarkbale activity against A2780 ovarian cancer cells.</p>
Subject(s)
Humans , Annona , Chemistry , Antineoplastic Agents, Phytogenic , Pharmacology , Cell Line, Tumor , Plant Bark , ChemistryABSTRACT
Objective To establish a new method for the identification of Cortex Phellodendri. Methods A HPLC fingerprint at 220 nm has been established for identification of Cortex Phellodendri, using a Merck-Lichrospher RP-C_(18) column(4.6?250mm, 5?m) and ACN(A) with the buffer of 0.3% H_3PO_4-NH(CH_2CH_3)_2 (B) in gradient as the mobile phase. The results were compared with the chromatograms of three species of Rbizoma Coptidis under the same conditions. Results HPLC fingerprint of Cortex Phellodendri at 220nm consists of 14 specific peaks. The steady appearance of the peaks and their relative contents were considered as important signs for the identification of this crude drug. The chromatograms of Rhizoma Coptidis were obviously different from the fingerprint of Cortex Phellodendri. Conclusion This method has been proven to be feasible for the identification of Cortex Phellodendri.
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Objective To establish a method for determination of Berberine and Phellodendrine in Cortex Phellodendri(CP).Methods Reverse-phase HPLC method was used for determination of Berberine and Phellodendrine in different batches of CP.The chromatographic conditions were:Merck-lichrospher RP-C18 column(4.6?250 mm,5 ?m),flow rate being 0.8 mL/min,column temperature at 25 ℃,detection wavelength at 284nm for Phellodendrine and 245nm for Berberine.Results The content of Berberine was 4 times and that of Phellodendrine in Chuan CP 2～3 times as much as that of Guan CP.Conclusion The method is proved to be feasible for quality assessment of Cortex Phellodendri.Limited contents of Berberine and Phellodendrine should be laid out for the great difference in Chuan CP and Guan CP.
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Objective To supply the basic research material for the optimal collecting time of Herba Andrographis. Methods A RP - HPLC method was used for the determination of Andrographolide and 14 - Deoxy - 11, 12 - didehy-droandrographolide from the stems and leaves of Herba Andrographis (cultured under GAP) in various growing periods on a Lichrospher RP - C18 (4. 6 mm? 250 mm, 5?m) column. The mobile phase was methanol - water (60:40), and the detection wavelength were set at 226 nm and 254 nm respectively. Results In different growing periods , contents of Andrographolide and 14 - Deoxy-11, 12 - didehydroandrographolide were higher in the sample collected in August and September. And for the same batch of sample, the contents in leaves are higher than those in the stems. Conclusion The phenophase from staminal time to pre - flowering period is the optimal collecting time for this herbal medicine, and leaves as medical part will be better than other parts.
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Objective To study the influences of polysacch arides(PS)from Radix Codonopsis on immune function and hematopoiesis in mice.Methods Several experiments have been condu cted for detecting delayed type hypersensitivity(DTH),hemolysin antibody concentration,endogenous spleen colony,3 H-TdRpermeate nuleated cells in bon e marrow.Results By oral administration,PS from Radix Codonopsis can promote the humoral immunity and PS at a small dosage en-hance the cellular immunity.PS from Radix Codonopsis can also elevate the hemoglobin cont ent of the hemolysis -induced blood -deficiency mice and increase the formation of endogenous spleen c olony,but has less effect on DNA synt hesis in the bone marrowof blood -deficiency mic e caused by 60 Co -?ray.Conclusion PS from Radix Codonopsis can enhance the im-mune function and improve the compen satory hematopoiesis of spleen and t heir mechanisms need to be further studied.